HPLC Column, Bidentate C8, 4um, 2.1mm ID x 100mm Length, 100A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40008‑10P‑2 Cogent Bidentate C8™ HPLC Column is a narrow‑bore, extended‑length analytical column designed for applications requiring greater resolution than 50 mm formats, while maintaining alternative reversed‑phase selectivity when C18 retention is excessive. Built on Cogent TYPE‑C™ silica hydride technology, this column delivers excellent selectivity, faster equilibration, and extremely robust column lifetimes compared with conventional silica‑based C8 columns.
The stationary phase consists of an aliphatic C8 hydrocarbon chain bonded via two direct silicon–carbon bonds (bidentate attachment) to a silica hydride surface. This chemistry produces a phase that is more hydrophobic than traditional C8 columns, yet less hydrophobic than Bidentate C18™, offering a controlled middle ground for method development. Unlike standard C8 phases, this column can also retain select hydrophilic compounds if desired and is stable at very low pH (below pH 2) without damage.
The 2.1 mm internal diameter supports low‑flow operation, reduced solvent consumption, and enhanced detector sensitivity, while the 100 mm length increases plate count and resolving power relative to 50 mm formats. As a result, this column is very popular for LC‑MS workflows when increased resolution is required without changing stationary phase chemistry.
Why Choose the 2.1 × 100 mm Bidentate C8™ Format
This column size is commonly selected when:
C18 columns provide excessive retention or long run times
Standard C8 columns do not deliver adequate resolution
Additional separation of closely eluting analytes is required
LC‑MS sensitivity and solvent‑use reduction are important
The extended length enhances resolution, while TYPE‑C™ silica hydride chemistry ensures fast equilibration and long service life.
Key Features
Cogent TYPE‑C™ Silica Hydride Technology
Non‑traditional reversed‑phase surface chemistry
Reduced silanol activity and secondary interactions
Faster equilibration than conventional silica‑based C8 columns
Extremely robust and reproducible column performance
Bidentate C8™ Bonding
Two‑point attachment via direct Si–C bonds
Balanced hydrophobic retention
Alternative selectivity when C18 is overly retentive
High‑Resolution Narrow‑Bore Geometry
2.1 mm ID × 100 mm length
Higher resolution than 50 mm formats
Reduced solvent consumption
LC‑MS‑Ready Format
Optimized for low‑flow operation
Enhanced sensitivity for ESI‑MS and LC‑MS/MS
Requires precise low‑flow pumping and reduced‑volume flow cells
System Requirements
For optimal performance with this narrow‑bore column:
Use a pump capable of accurate and reproducible low‑flow delivery
Employ detector flow cells designed for low internal volumes
Typical Applications
LC‑MS and LC‑MS/MS
Hydrophobic and moderately polar compounds
Situations where C18 retention is excessive
High‑resolution reversed‑phase methods
Method development and optimization